Environmental Responses in Plants by Paula Duque

Author:Paula Duque
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

5.One hour after plating seeds onto MS medium transfer the plates to a dark room. Illuminate one plate with far-red light (FR) of intensity of 3.2–5 μmol/m2/s for 5 min (see Note 4 ). As a control for light-induced germination, illuminate the second plate first with FR pulse of 5 min (3.2 μmol/m2/s) and then immediately illuminate the same plate with red (R) light (20 μmol/m2/s) for 5 min.

6.Wrap MS medium plates in aluminum foil ensuring that day light is not penetrating into the plates and incubate plates in a growth chamber under normal germination conditions.

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